miscript rt kit Search Results


miscript sybr green pcr kit  (Qiagen)
96
Qiagen miscript sybr green pcr kit
Miscript Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription kit  (Qiagen)
96
Qiagen reverse transcription kit
Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript mirna pcr system  (Qiagen)
99
Qiagen miscript mirna pcr system
Miscript Mirna Pcr System, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript single cell qpcr kit  (Qiagen)
97
Qiagen miscript single cell qpcr kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Single Cell Qpcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/Cell+Lysis+Solution/pm33420081-403-5-11
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miscript ii rt kit  (Qiagen)
99
Qiagen miscript ii rt kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Ii Rt Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/RNase+A/pmc07716711-83-5-9
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miscript reverse transcription kit  (Qiagen)
97
Qiagen miscript reverse transcription kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/Reverse+transcriptase/pm28753793-80-7-11
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rnase free water  (Qiagen)
99
Qiagen rnase free water
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Rnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/RNase-Free+Water/10__1186_slash_s12645___025___00331___4-94-29-36
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miscript ii rt kit  (Bio-Rad)
96
Bio-Rad miscript ii rt kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Ii Rt Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/miscript+rt+kit/Bio-Rad+Protein+Assay+Kit+II/pmc12650977-119-6-24
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fc 131 1096 miscript ii rt kit qiagen  (Illumina Inc)
99
Illumina Inc fc 131 1096 miscript ii rt kit qiagen
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Fc 131 1096 Miscript Ii Rt Kit Qiagen, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d RT-qPCR analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.

Journal: Nature communications

Article Title: SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression.

doi: 10.1038/s41467-020-20460-1

Figure Lengend Snippet: Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d RT-qPCR analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: This assay was performed with miScript Single Cell qPCR Kit (331055, Qiagen) according to the manufacturer’s instructions.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Gene Expression, Clone Assay, CRISPR, Two Tailed Test