miscript rt kit Search Results


miscript sybr green pcr kit  (Qiagen)
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Qiagen miscript sybr green pcr kit
Miscript Sybr Green Pcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription kit  (Qiagen)
96
Qiagen reverse transcription kit
Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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reverse transcription kit - by Bioz Stars, 2026-08
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miscript mirna pcr system  (Qiagen)
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Qiagen miscript mirna pcr system
Miscript Mirna Pcr System, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript single cell qpcr kit  (Qiagen)
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Qiagen miscript single cell qpcr kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Single Cell Qpcr Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript ii rt kit  (Qiagen)
99
Qiagen miscript ii rt kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Ii Rt Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript reverse transcription kit  (Qiagen)
97
Qiagen miscript reverse transcription kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Reverse Transcription Kit, supplied by Qiagen, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rnase free water  (Qiagen)
99
Qiagen rnase free water
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Rnase Free Water, supplied by Qiagen, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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miscript ii rt kit  (Bio-Rad)
96
Bio-Rad miscript ii rt kit
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Miscript Ii Rt Kit, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fc 131 1096 miscript ii rt kit qiagen  (Illumina Inc)
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Illumina Inc fc 131 1096 miscript ii rt kit qiagen
Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d <t>RT-qPCR</t> analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.
Fc 131 1096 Miscript Ii Rt Kit Qiagen, supplied by Illumina Inc, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d RT-qPCR analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.

Journal: Nature communications

Article Title: SLE non-coding genetic risk variant determines the epigenetic dysfunction of an immune cell specific enhancer that controls disease-critical microRNA expression.

doi: 10.1038/s41467-020-20460-1

Figure Lengend Snippet: Fig. 6 Targeting rs2431697 intervenes with the activation of the interferon pathway by modulating miR-146a expression. a–d RT-qPCR analysis of interferon-inducible gene expression in U-937 WT and deletion clones after treating with Interferon-α (IFIT1: **P = 0.0067, **IFIT3: P = 0.0027, **OAS1: P = 0.0066, ***IFI44: P = 0.0003) (n = 6, replicates represent unique biological clone replicates). e Flow scheme of inhibiting SLE IFN pathway by CRISPR activation (CRIPSRa) approach. f RT-qPCR analysis of Pri-miR-146a expression in SLE patients’ PBMCs after treating with the CRISPRa system targeting the rs2431697 locus, *P = 0.0204 (n = 9, replicates represent biological samples from unique individuals). g CRISPRa therapy decreases the IFN score of SLE patients’ PBMCs based on sgRNAs targeting the rs2431697 site, *P = 0.0158 (n = 9, replicates represent biological samples from unique individuals). Data are represented as mean ± SEM, and P-values are calculated using unpaired two-tailed Student’s t-test (a–d) and paired two-tailed Student’s test (f, g). *P < 0.05; **P < 0.01; ***P < 0.001.

Article Snippet: This assay was performed with miScript Single Cell qPCR Kit (331055, Qiagen) according to the manufacturer’s instructions.

Techniques: Activation Assay, Expressing, Quantitative RT-PCR, Gene Expression, Clone Assay, CRISPR, Two Tailed Test